Anti-Transglutaminase 2抗体(ab421)


  • 产品名称Anti-Transglutaminase 2抗体
    参阅全部 Transglutaminase 2 一抗
  • 描述
    兔多克隆抗体to Transglutaminase 2
  • 特异性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
  • 经测试应用适用于: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
  • 免疫原

    Full length native protein (purified) (Guinea Pig).

  • 阳性对照
    • IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate



Our Abpromise guarantee covers the use of ab421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/5000.
ELISA 1/300. 1/300 (See Abreview).
IHC-P 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration. PubMed: 21359202
ICC/IF 1/1000.


  • 功能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody 图像

  • This was submitted as part of a review by Dr O Fromigue

  • ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-Transglutaminase 2 antibody (ab421)参考文献

This product has been referenced in:
  • Petersen-Jones HG  et al. Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls. Am J Physiol Heart Circ Physiol 308:H592-602 (2015). Read more (PubMed: 25599570) »
  • Sánchez-Lara AC  et al. Feline Chronic Kidney Disease Is Associated With Upregulation of Transglutaminase 2: A Collagen Cross-Linking Enzyme. Vet Pathol N/A:N/A (2014). Cat . Read more (PubMed: 25047228) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (lung)
Specification lung
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%


Verified customer

提交于 Aug 08 2005

Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further quest...

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This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the a...

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