Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 15 µl antibody per 500-1000 µg protein extract from mouse liver or spleen extract. Does not IP from mouse kidney extract.
WB: 1/1000. Detects a band of approximately 78 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-Transglutaminase 2 antibody 图像
Western blot - Anti-Transglutaminase 2 antibody (ab10445)
All lanes :
Anti-Transglutaminase 2 antibody (ab10445) at 1/1000 dilution
Lane 1 :
Membrane lysates from wild-type primary human osteoblasts (HOB)
Lane 2 :
Membrane lysates from TG2-transfected HOB cells (HOB-TG14)
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique
Performed under reducing conditions.
Image from Scarpellini A et al, J Biol Chem. 2009 Jul 3;284(27):18411-23. Epub 2009 Apr 27, Fig 3. DOI 10.1074/jbc.M109.012948
Anti-Transglutaminase 2 antibody (ab10445)参考文献
This product has been referenced in:
et al. Heparan Sulfate Proteoglycans Are Receptors for the Cell-surface Trafficking and Biological Activity of Transglutaminase-2. J Biol Chem 284:18411-23 (2009).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"