Anti-Transferrin Receptor抗体[MEM-189] (ab1086)

概述

  • 产品名称
    Anti-Transferrin Receptor抗体[MEM-189]
    参阅全部 Transferrin Receptor 一抗
  • 描述
    小鼠单克隆抗体[MEM-189] to Transferrin Receptor
  • 宿主
    Mouse
  • 特异性
    The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
  • 经测试应用
    适用于: IHC-FrFl, Flow Cyt, IP, WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Transferrin Receptor.

性能

应用

Our Abpromise guarantee covers the use of ab1086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • 疾病相关
      Immunodeficiency 46
    • 序列相似性
      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • 翻译后修饰
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • 细胞定位
      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    图片

    • ab1086 staining Transferrin Receptor in Human PC-3 prostate cancer cell line by Flow Cytometry. The sample was incubated with the primary antibody (0.3µg/ml in PBS + 2% FBS) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
      Gating Strategy: Cells gated for live and singlets.

      See Abreview

    文献

    This product has been referenced in:
    • Yang X  et al. Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2. J Clin Invest 127:1505-1516 (2017). Read more (PubMed: 28287409) »
    • Zhao M  et al. Iron-induced neuronal damage in a rat model of post-traumatic stress disorder. Neuroscience 330:90-9 (2016). WB, IHC-Fr ; Rat . Read more (PubMed: 27208615) »

    See all 11 Publications for this product

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 27 2017

    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Human Cell (PC-3 Prostrate cancer cell line)
    Specification
    PC-3 Prostrate cancer cell line
    Gating Strategy
    Cells gated for live and singlets
    Preparation
    Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
    Sample buffer: PBS + 2% FBS
    Username

    Abcam user community

    Verified customer

    提交于 Nov 05 2013

    Thank you for contacting us.

    According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual p...

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    Thank you for contacting us.

    Unfortunately the epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

    I am sorry for not being able to provide any useful info...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Thank you for contacting us.

    Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

    http://www.abcam.com/index.html?pageconfig=resou...

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    Thank you for your enquiry.

    We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivit...

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    Thank you for bringing that to my attention.
    I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab...

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    Thank you for your response.

    I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience t...

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    I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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