Anti-Transferrin Receptor抗体[DF1513] (Biotin) (ab7350)

概述

  • 产品名称Anti-Transferrin Receptor抗体[DF1513] (Biotin)
    参阅全部 Transferrin Receptor 一抗
  • 描述
    小鼠单克隆抗体[DF1513] to Transferrin Receptor (Biotin)
  • 偶联物Biotin
  • 特异性DF1513 recognises the human CD71 cell surface antigen, a 190 kD homodimeric glycoprotein.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Rhesus monkey
  • 免疫原

    KG1 acute myeloid leukemia cell line

性能

应用

Our Abpromise guarantee covers the use of ab7350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow cytometry: neat. Use 10 µl to label 106 cells.
    Does not work in immunohistology on frozen and paraffin-embedded sections.
  • 靶标

    • 功能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • 疾病相关Immunodeficiency 46
    • 序列相似性Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • 翻译后修饰N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • 细胞定位Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Anti-Transferrin Receptor antibody [DF1513] (Biotin) (ab7350)参考文献

    This product has been referenced in:
    • Ayala P  et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). Read more (PubMed: 16309460) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. Ab8598 and Ab7350 are the same clone, DF1513. We get these two antibodies from two different sources. Ab8598 has been tested for application in IHC-frozen sections. Unfortunately, we are not aware of any publications that fe...

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    Thank you for your enquiry. Any human cell line eg. KG1, U266, MOLT4 (i.e. anything that is proliferating well) would represent a suitable positive control for this antibody in flow cytometry.

    As we would not have tested the anti-human CD71 antibody in cell monolayers/smears we would be unable to guarantee its suitability for this procedure. However, having said that, fixing cells on slides and staining them is probably the most simple imm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"