Anti-Transferrin Receptor抗体[DF 1513 (Workshop IV)] (ab116015)

概述

  • 产品名称Anti-Transferrin Receptor抗体[DF 1513 (Workshop IV)]
    参阅全部 Transferrin Receptor 一抗
  • 描述
    小鼠单克隆抗体[DF 1513 (Workshop IV)] to Transferrin Receptor
  • 经测试应用适用于: WB, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Transferrin Receptor.

  • 阳性对照
    • Human tonsil and placenta tissues.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 15th June 2017.  The following lots are from ascites and still in stock as of 15th June 2017 (GR168460-1).  Lot numbers higher than GR168460-1 will be from tissue culture supernatant.  Please note that the dilutions may need to be adjusted accordingly.

性能

应用

Our Abpromise guarantee covers the use of ab116015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/200. Detects a band of approximately 90-95 kDa (predicted molecular weight: 85 kDa).
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

(methanol fixed).Use at an assay dependent concentration

靶标

  • 功能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • 疾病相关Immunodeficiency 46
  • 序列相似性Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • 翻译后修饰N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • 细胞定位Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [DF 1513 (Workshop IV)] (ab116015)参考文献

ab116015 has not yet been referenced specifically in any publications.

Product Wall

We do not have a CD71 antibody that has been tested in ELISA or any other kind of capture assay, but the monoclonal antibody ab116015 is specific for an extracellular epitope and can at least identify CD71 on cells. Capturing reticulocytes with the mag...

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Thank you for your email.

Unfortunately, no information about the use of a permeabilization step was found by the lab. Thus, it is not clear if the binding site is extra- or intracellular.

I am sorry to not have more helpfulinform...

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Thank you for contacting us.

I have heard back from the lab that the epitope of this antibody is not known. I have asked them to check if a permeabilization step is needed as this would tell us if the antibody binding site would be extracell...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"