Anti-Transferrin抗体(HRP) (ab9036)

概述

  • 产品名称
    Anti-Transferrin抗体(HRP)
    参阅全部 Transferrin 一抗
  • 描述
    羊多克隆抗体to Transferrin (HRP)
  • 偶联物
    HRP
  • 特异性
    The unconjugated immunoglobulin gives a single arc when tested by immunoelectrophoresis and 2D-IEPagainst human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti-humantransferrin of known specificity.
  • 经测试应用
    适用于: ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The immunogen used was Human Holo-Transferrin purified from human serum and shown to be homogeneousby SDS-PAGE.

性能

应用

Our Abpromise guarantee covers the use of ab9036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明
    For enzyme immunoassays, to give an A450nm = 1.0 using wells coated with transferrin at 2µg/mLand TMB as a substrate: 1/12800 – 1/25600
    The titres reported should be used as guidelines for determining the working dilution in the userssystem.

    The following points should be observed when using this product:

    1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2)
    2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers.Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate.
    3) Working dilutions of the conjugate should be prepared immediately prior to use.
    4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided.
    5) The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.
  • 靶标

    • 功能
      Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
    • 组织特异性
      Expressed by the liver and secreted in plasma.
    • 疾病相关
      Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
    • 序列相似性
      Belongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    文献

    ab9036 has not yet been referenced specifically in any publications.

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