Anti-Transferrin抗体(Biotin) (ab34673)

概述

  • 产品名称
    Anti-Transferrin抗体(Biotin)
    参阅全部 Transferrin 一抗
  • 描述
    兔多克隆抗体to Transferrin (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: WB, Dot blot, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Transferrin from Human serum.

  • 常规说明

    10-20 Biotin molecules per rabbit IgG molecule.

性能

应用

Our Abpromise guarantee covers the use of ab34673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
Dot blot Use at an assay dependent concentration.
ELISA 1/20000 - 1/150000.

This antibody has been assayed against 1.0µg of Transferrin in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

靶标

  • 功能
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 疾病相关
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

图片



  • Performed under reducing conditions.

    Predicted band size : 77 kDa

    Multiplex western blot analysis of 35 µg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.

  • Anti-Transferrin antibody (Biotin) (ab34673) at 1/1000 dilution + Human depleted serum with Blocking buffer

    Secondary
    Peroxidase streptavidin conjugate at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size : 77 kDa


  • Performed under reducing conditions.

    Predicted band size : 77 kDa

    Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.

实验方案

文献

ab34673 has not yet been referenced specifically in any publications.

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