概述

  • 产品名称Anti-Transferrin抗体
    参阅全部 Transferrin 一抗
  • 描述
    兔多克隆抗体to Transferrin
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human transferrin, partially iron saturated.

  • 阳性对照
    • Human serum transferrin. IF/ICC: HepG2 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab66952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/150000 - 1/300000. Coating antigen 800ng/well
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody 图像

  • Anti-Transferrin antibody (ab66952) at 1/50000 dilution + human serum transferrin at 1.5 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ICC/IF image of ab66952 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66952, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab66952, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Transferrin antibody (ab66952)参考文献

This product has been referenced in:
  • Di Luca A  et al. 2D DIGE proteomic analysis of early post mortem muscle exudate highlights the importance of the stress response for improved water-holding capacity of fresh pork meat. Proteomics 13:1528-44 (2013). Read more (PubMed: 23456991) »
  • Pitek AS  et al. Transferrin coated nanoparticles: study of the bionano interface in human plasma. PLoS One 7:e40685 (2012). Read more (PubMed: 22829881) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab66952.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"