概述

  • 产品名称Anti-Transferrin抗体
    参阅全部 Transferrin 一抗
  • 描述
    山羊多克隆抗体to Transferrin
  • 经测试应用适用于: IHC-P, ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照
    • IHC-P: human tissue liver sections

性能

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应用

Our Abpromise guarantee covers the use of ab19177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/100 - 1/1000. 1000 - 1/30000 (primary)
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa. 1/1000 - 1/30000 (Chemiluminescent detection)
ICC 1/200 - 1/2000.

靶标

  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody 图像

  • IHC image of Transferrin staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19177, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Transferrin antibody (ab19177)参考文献

ab19177 has not yet been referenced specifically in any publications.

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