Anti-Transferrin抗体[11D3] (HRP) (ab10208)


  • 产品名称Anti-Transferrin抗体[11D3] (HRP)
    参阅全部 Transferrin 一抗
  • 描述
    小鼠单克隆抗体[11D3] to Transferrin (HRP)
  • 偶联物HRP
  • 经测试应用适用于: ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Placental transferrin receptor or transferrin.

  • 阳性对照
    • human brain
  • 常规说明Concentration varies from lot to lot and can be provided on request.



Our Abpromise guarantee covers the use of ab10208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8B9] to Transferrin (ab10212). Can be used as both coating and conjugate antibody when paired with recommended pair for detection of transferrin.
WB 1/500. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).


  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody [11D3] (HRP) 图像

  • All lanes : Anti-Transferrin antibody [11D3] (HRP) (ab10208) at 1/500 dilution

    Lane 1 : human brain
    Lane 2 : human brain
    Lane 3 : human brain
    Lane 4 : human brain
    Lane 5 : human brain
    Lane 6 : human brain
    Lane 7 : human brain
    Lane 8 : human brain
    Lane 9 : human brain

    Lysates/proteins at 20 µg per lane.

    DAKO Cytomation Conjugation: HRP at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : many <77 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Anti-Transferrin antibody [11D3] (HRP) (ab10208)参考文献

This product has been referenced in:
  • Shakor AB  et al. Sphingomyelin Synthase 1-generated Sphingomyelin Plays an Important Role in Transferrin Trafficking and Cell Proliferation. J Biol Chem 286:36053-62 (2011). Read more (PubMed: 21856749) »
  • Grønborg M  et al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5:157-71 (2006). Read more (PubMed: 16215274) »

See all 3 Publications for this product

Product Wall

According to the testing lab, the sELISA was performed on these conditions:

Capture Ab10212: 10 μg/ml for plate coating

Detection Ab10208: 1.5 μg/ml
Human transferrin antigen – different concentrations

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain lysate)
Specification Brain lysate
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 02 2009

Thank you for your enquiry. We would recommend the typical ELISA coating dilutions which we start with: 50 uL/well of a 2 ug/mL solution. The tracer antibody dilution depends on the conjugates recommended dilution range. Hope this helps.