Anti-Transcription termination factor 1抗体(ab87726)

概述

  • 产品名称
    Anti-Transcription termination factor 1抗体
    参阅全部 Transcription termination factor 1 一抗
  • 描述
    兔多克隆抗体to Transcription termination factor 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Transcription termination factor 1 (CAA58807.1)

  • 阳性对照
    • HeLa or 293T cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.

靶标

  • 功能
    Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
  • 序列相似性
    Contains 2 Myb-like domains.
  • 结构域
    The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RNA polymerase I termination factor antibody
    • Transcription termination factor 1 antibody
    • Transcription termination factor I antibody
    • Transcription termination factor RNA polymerase I antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF-I antibody
    • Ttf1 antibody
    • TTF1_HUMAN antibody
    see all

图片

  • All lanes : Anti-Transcription termination factor 1 antibody (ab87726) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : ~118 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

实验方案

文献

ab87726 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for keeping in touch.

Please take my apologies for the long delay of my reply. I have contacted the originators of some of our relevant antibodies in order to get a confirmation (mistakes can happen, especially with those similar ta...

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