The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 98 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
序列相似性Contains 2 Myb-like domains.
结构域The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.