Anti-Transcription factor 25抗体(ab96813)

概述

  • 产品名称
    Anti-Transcription factor 25抗体
    参阅全部 Transcription factor 25 一抗
  • 描述
    兔多克隆抗体to Transcription factor 25
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 427 and 648 of Transcription factor 25 (NP_055787)

  • 阳性对照
    • Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab96813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
  • 组织特异性
    In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
  • 序列相似性
    Belongs to the TCF25 family.
  • 结构域
    The C-terminal region mediates transcriptional repression.
  • 细胞定位
    Nucleus. Some staining in the cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FKSG26 antibody
    • hKIAA1049 antibody
    • Hulp1 antibody
    • KIAA1049 antibody
    • Nuclear localized protein 1 antibody
    • NULP1 antibody
    • PRO2620 antibody
    • TCF-25 antibody
    • Tcf25 antibody
    • TCF25_HUMAN antibody
    • Transcription factor 25 (basic helix loop helix) antibody
    • Transcription factor 25 antibody
    see all

图片

  • Anti-Transcription factor 25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 77 kDa

    7.5% SDS Page

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.

文献

ab96813 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Loading amount
40 µg
Specification
HeLa cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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