Anti-TCF-25抗体(ab96813)
Key features and details
- Rabbit polyclonal to TCF-25
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TCF-25抗体
参阅全部 TCF-25 一抗 -
描述
兔多克隆抗体to TCF-25 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow -
免疫原
Recombinant fragment corresponding to Human TCF-25 aa 427-648.
Database link: NP_055787 -
阳性对照
- Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96813于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 77 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
|
说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 77 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development. -
组织特异性
In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart. -
序列相似性
Belongs to the TCF25 family. -
结构域
The C-terminal region mediates transcriptional repression. -
细胞定位
Nucleus. Some staining in the cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 618386 Cow
- Entrez Gene: 22980 Human
- Entrez Gene: 66855 Mouse
- Omim: 612326 Human
- SwissProt: Q9BQ70 Human
- SwissProt: Q8R3L2 Mouse
- Unigene: 415342 Human
- Unigene: 178818 Mouse
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别名
- FKSG26 antibody
- hKIAA1049 antibody
- Hulp1 antibody
see all
图片
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Anti-TCF-25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 77 kDa7.5% SDS Page
Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab96813 尚未被引用在任何文献中。