The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The specific function of TRAK1 remains unknown. It interacts with O-GlcNAc transferase, RHOT1/Miro-1 and RHOT2/Miro-2. It shows high expression in spinal cord and moderate expression in all other tissues and specific brain regions examined.