概述

  • 产品名称Anti-TRAIL抗体
    参阅全部 TRAIL 一抗
  • 描述
    兔多克隆抗体to TRAIL
  • 经测试应用适用于: ELISA, Neutralising, WB, IHC-P, ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hTRAIL (human TNF-Related Apoptosis Inducing Ligand)

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231

性能

应用

Our Abpromise guarantee covers the use of ab9959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. To detect hTRAIL by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTRAIL.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL (30 ng/ml), a concentration of 0.5 - 0.8 mg/ml of this antibody is required.
WB Use at an assay dependent concentration. To detect hTRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Sandwich ELISA Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab12124 as Capture.

靶标

  • 功能Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • 组织特异性Widespread; most predominant in spleen, lung and prostate.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo 2 ligand antibody
    • APO 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF10 antibody
    • TRAIL antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all

Anti-TRAIL antibody 图像

  • ICC/IF image of ab9959 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9959 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.
  • ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

实验方案

Anti-TRAIL antibody (ab9959)参考文献

This product has been referenced in:
  • Graves JD  et al. Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity. Cancer Cell 26:177-89 (2014). WB ; Human . Read more (PubMed: 25043603) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. I am pleased to help answer your questions. 1. I am sorry to confirm that ab12124 antibody would not be suitable for detection of the ab78818 protein. As you have noticed from the different amino acid sequenc...

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