Anti-TRAIL抗体(ab65121)
Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRAIL抗体
参阅全部 TRAIL 一抗 -
描述
兔多克隆抗体to TRAIL -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide from internal of human CD253.
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阳性对照
- Extracts from 293 cells and HUVEC cells, and A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65121于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/500 - 1/1000.
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WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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说明 |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
靶标
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功能
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
组织特异性
Widespread; most predominant in spleen, lung and prostate. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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别名
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
图片
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These images show A549 cells, stained with ab65121 at 1/500 - 1/1000 dilution; with and without the immunising peptide.
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All lanes : Anti-TRAIL antibody (ab65121) at 1/500 dilution
Lane 1 : extracts from 293 cells
Lane 2 : extracts from Huvec cells
Lane 3 : extracts from Huvec cells with immunising peptide
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: ~48 kDa, ~60 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab65121 被引用在 1 文献中.
- Ru Q et al. Preclinical study of rAAV2-sTRAIL: pharmaceutical efficacy, biodistribution and safety in animals. Cancer Gene Ther 24:251-258 (2017). PubMed: 28429751