RabMAb

Anti-TRAF6抗体[EP591Y] (ab33915)

概述

  • 产品名称Anti-TRAF6抗体[EP591Y]
    参阅全部 TRAF6 一抗
  • 描述
    兔单克隆抗体[EP591Y] to TRAF6
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Zebrafish
  • 免疫原

    A synthetic peptide corresponding to residues near the N term of TRAF6 (Human)

  • 阳性对照
    • Jurkat cell lysate, Human colon adenocarcinoma
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab33915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.Can be blocked with TRAF6 peptide (ab183540).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
    • 组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the TNF receptor-associated factor family. A subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 2 TRAF-type zinc fingers.
    • 结构域The coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.
    • 翻译后修饰Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
      Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
    • 细胞定位Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E3 ubiquitin-protein ligase TRAF6 antibody
      • Interleukin 1 signal transducer antibody
      • Interleukin-1 signal transducer antibody
      • MGC 3310 antibody
      • MGC:3310 antibody
      • MGC3310 antibody
      • OTTHUMP00000232772 antibody
      • OTTHUMP00000232773 antibody
      • RING finger protein 85 antibody
      • RNF 85 antibody
      • RNF85 antibody
      • TNF receptor associated factor 6 antibody
      • TNF receptor-associated factor 6 antibody
      • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
      • TRAF 6 antibody
      • Traf6 antibody
      • TRAF6_HUMAN antibody
      see all

    Anti-TRAF6 antibody [EP591Y] 图像

    • Anti-TRAF6 antibody [EP591Y] (ab33915) at 1/10000 dilution + Jurkat cell lysate

      Predicted band size : 58 kDa
    • Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-TRAF6 (ab33915)
    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling TRAF6 with purified ab33915 at 1/240 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • ab33915 staining TRAF6 in Human platelet cells by Flow cytometry.
      Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

      P : Permeabilized
      US : Unstained (Red Peak)
      IGG RB : IgG Rabbit (Blue Peak)
      TRAF6 Ab (Green Peak)

      See Abreview

    Anti-TRAF6 antibody [EP591Y] (ab33915)参考文献

    This product has been referenced in:
    • Meininger I  et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Read more (PubMed: 27068814) »
    • Padrão AI  et al. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia. Arch Biochem Biophys 567:13-21 (2015). Read more (PubMed: 25575785) »

    See all 12 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Cell lysate - whole cell (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 17 2014

    Application Western blot
    Loading amount 7.5 µg
    Gel Running Conditions Non-reduced Denaturing (7.5%)
    Sample Zebrafish Tissue lysate - whole (embryos 5-7 days post fertilization)
    Specification embryos 5-7 days post fertilization
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Rubén

    Verified customer

    提交于 Oct 22 2013

    Thank you for contacting Abcam and for your interest in ab33915.


    I can confirm for you that this is indeed a rabbit monoclonal antibody. Rabbit antibodies are generally high affinity and are an ideal species to choose for making antibod...

    Read More

    Thank you for your reply.

    Our IRAK antibody ab238 will be compatible with any of our EasyLink conjugation kits. The full list of available sizes and fluorophores can be found at http://www.abcam.com/EasyLink, but for example you could use the...

    Read More

    Thank you for contacting us.

    We do not currently have any ELISA kits to TRAF6, IRAK1, or IRF5. We currently have three ELISA kits for STAT1 phosphorylations, but none to total STAT1:

    ab119649: PhosphoTracer STAT1 (pY701) ELISA Kit <...

    Read More

    Thank you for contacting us. We currently have one antibody for each of these targets that has been validated for use in Flow Cytometry:

    ab238 rabbit polyclonal to IRAK; tested for use in WB, IP, IHC-P, Flow Cyt, ICC/IF; reacts with human, mo...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa Cells)
    Loading amount 25 µg
    Specification HeLa Cells
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 01 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Platelets)
    Loading amount 20 µg
    Specification Platelets
    Treatment ADP for 30 min
    Gel Running Conditions Reduced Denaturing (4-20%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    提交于 Nov 23 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (Platelets)
    Specification Platelets
    Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Gating Strategy Platelets
    Username

    Abcam user community

    Verified customer

    提交于 Dec 21 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (RAW264.7)
    Loading amount 50000 cells
    Specification RAW264.7
    Treatment 100 ng/ml recombinant mouse RANKL
    Gel Running Conditions Reduced Denaturing (NuPage 10% Bis-Tris)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 01 2010

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"