Anti-TRAF6抗体(ab137452)
Key features and details
- Rabbit polyclonal to TRAF6
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TRAF6抗体
参阅全部 TRAF6 一抗 -
描述
兔多克隆抗体to TRAF6 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 144-408 of Human TRAF6 (UniProt ID: Q9Y4K3).
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阳性对照
- WB: A431, A549, HeLa, HepG2,NIH/3T3, BCL-1, PC-12, Rat2 cell lysate. IHC-P: 59T xenograft, mouse lung and rat brain tissue. IF: HeLa cells. IP: K562 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. -
组织特异性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. -
结构域
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
翻译后修饰
Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. -
细胞定位
Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7189 Human
- Entrez Gene: 22034 Mouse
- Entrez Gene: 311245 Rat
- Omim: 602355 Human
- SwissProt: Q9Y4K3 Human
- SwissProt: P70196 Mouse
- SwissProt: B5DF45 Rat
- Unigene: 591983 Human
see all -
别名
- E3 ubiquitin-protein ligase TRAF6 antibody
- Interleukin 1 signal transducer antibody
- Interleukin-1 signal transducer antibody
see all
图片
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Anti-TRAF6 antibody (ab137452) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 60 kDa10% SDS-PAGE
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Paraffin-embedded 59T xenograft tissue stained TRAF6 using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TRAF6 at cytoplasm and nucleus (green) with ab137452 at 1/100 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution. Blue: Hoechst 33342 staining.
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IP samples: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate.
1- Control with 4 μg of preimmune Rabbit IgG;
2- Immunoprecipitation of TRAF6 protein by 4 μg ab137452.7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by ab137452 at 1/500 dilution, followed by anti-rabbit IgG antibody.
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Paraffin-embedded mouse lung tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
7.5% SDS PAGE -
Paraffin-embedded rat brain tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa7.5% SDS PAGE
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All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa7.5% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab137452 被引用在 14 文献中.
- Wan P et al. ASB17 Facilitates the Burst of LPS-Induced Inflammation Through Maintaining TRAF6 Stability. Front Cell Infect Microbiol 12:759077 (2022). PubMed: 35174103
- Kim JS et al. PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6. Front Immunol 13:862628 (2022). PubMed: 35572598
- Sun W et al. Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction. Nat Commun 13:2899 (2022). PubMed: 35610255
- Zhou Q et al. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model. Open Med (Wars) 17:907-914 (2022). PubMed: 35647304
- Peng K et al. Alpha-Momorcharin Inhibits Proinflammatory Cytokine Expression by M1 Macrophages but Not Anti-Inflammatory Cytokine Expression by M2 Macrophages. J Inflamm Res 15:4853-4872 (2022). PubMed: 36042868