重组
RabMAb

Anti-TRAF4抗体[EPR1729] (ab108991)

概述

  • 产品名称
    Anti-TRAF4抗体[EPR1729]
    参阅全部 TRAF4 一抗
  • 描述
    兔单克隆抗体[EPR1729] to TRAF4
  • 经测试应用
    适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Jurkat, HeLa and SKBR-3 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能
      Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
    • 组织特异性
      Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
    • 序列相似性
      Belongs to the TNF receptor-associated factor family. B subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 3 TRAF-type zinc fingers.
    • 结构域
      The coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.
    • 翻译后修饰
      Polyubiquitinated, leading to its proteasomal degradation.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CART 1 antibody
      • CART1 antibody
      • cysteine rich domain associated with ring and TRAF domain antibody
      • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
      • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
      • Malignant 62 antibody
      • Metastatic lymph node gene 62 protein antibody
      • MLN 62 antibody
      • MLN62 antibody
      • RING finger protein 83 antibody
      • RNF 83 antibody
      • RNF83 antibody
      • TNF receptor associated factor 4 antibody
      • TNF receptor-associated factor 4 antibody
      • TRAF 4 antibody
      • Traf4 antibody
      • TRAF4_HUMAN antibody
      • tumor necrosis receptor-associated factor 4A antibody
      see all

    Anti-TRAF4 antibody [EPR1729] 图像

    • Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 54 kDa

    Anti-TRAF4 antibody [EPR1729] (ab108991)参考文献

    This product has been referenced in:
    • Zepp JA  et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). Read more (PubMed: 22649194) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LnCaP cells)
    Loading amount
    50 µg
    Specification
    LnCaP cells
    Gel Running Conditions
    Reduced Denaturing (5-15% gradient gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    提交于 Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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