Anti-TRAF4抗体(ab88612)
Key features and details
- Mouse polyclonal to TRAF4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TRAF4抗体
参阅全部 TRAF4 一抗 -
描述
小鼠多克隆抗体to TRAF4 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human TRAF4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
阳性对照
- WB: human pancreas tissue lysate, TRAF4 transfected 293T cell lysate ICC/IF: HeLa cell
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88612于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
-
功能
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. -
组织特异性
Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. -
序列相似性
Belongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers. -
结构域
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
翻译后修饰
Polyubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 9618 Human
- Omim: 602464 Human
- SwissProt: Q9BUZ4 Human
- Unigene: 8375 Human
-
别名
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
see all
图片
-
Anti-TRAF4 antibody (ab88612) at 1 µg/ml + human pancreas tissue lysate at 50 µg
Predicted band size: 54 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TRAF4 antibody (ab88612) at 1 µg/ml
Lane 1 : TRAF4 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
ab88612, at 10 µg/ml, staining TRAF4 on HeLa cell by Immunofluorescence.
实验方案
数据表及文件
-
Datasheet download
文献 (2)
ab88612 被引用在 2 文献中.
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273
- Li J et al. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2. Cell Death Differ 26:2652-2666 (2019). PubMed: 31076633