概述
- 产品名称Anti-TRAF3抗体
参阅全部 TRAF3 一抗 - 描述兔多克隆抗体to TRAF3
- 经测试应用适用于: WBmore details
- 种属反应性与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
- 免疫原
Synthetic peptide corresponding to Human TRAF3 aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P36941 - 阳性对照
- This antibody gave a positive signal in HEK293 whole cell lysate as well as the following Human tissue lysates: Fetal Kidney; Fetal Liver; Fetal Lung; Thymus; Spleen; Lymph node; Bone marrow.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab110781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
靶标
- 功能Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
- 序列相似性Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. - 结构域The MATH/TRAF domain binds to receptor cytoplasmic domains.
The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing. - 翻译后修饰Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
- 细胞定位Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
- Information by UniProt
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数据库链接
- Entrez Gene: 7187 Human
- Omim: 601896 Human
- SwissProt: Q13114 Human
- Unigene: 510528 Human
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别名
- CAP 1 antibody
- CAP-1 antibody
- CAP1 antibody
see all
Anti-TRAF3 antibody 图像
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Western blot - Anti-TRAF3 antibody (ab110781)All lanes : Anti-TRAF3 antibody (ab110781) at 1 µg/ml
Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue
Lane 2 : Fetal Liver (Human) Normal Tissue Lysate
Lane 3 : Lung (Human) Whole Cell Lysate - fetal normal tissue
Lane 4 : Thymus (Human) Tissue Lysate - adult normal tissue
Lane 5 : Spleen (Human) Tissue Lysate - adult normal tissue
Lane 6 : Lymph node (Human) Tissue Lysate - adult normal tissue
Lane 7 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 8 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab1107881 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Anti-TRAF3 antibody (ab110781)参考文献
ab110781 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"