The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
1/100. Perform antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt.
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Found in all examined tissues.
Contains 1 death domain.
Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.