概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRA2B
  • 经测试应用
    适用于: WB, IHC-P, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
    预测可用于: Rabbit, Horse, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide: MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS, corresponding to amino acids 1-50 of Human TRA2B

  • 阳性对照
    • Jurkat cell lysate. Human lung tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22437831

靶标

Anti-TRA2B antibody 图像

  • All lanes : Anti-TRA2B antibody (ab66901) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRA2B with ab66901 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

Anti-TRA2B antibody (ab66901)参考文献

This product has been referenced in:
  • Llorian M  et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . Read more (PubMed: 27317697) »
  • Zheng X  et al. TRA2ß controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle. Am J Physiol Cell Physiol 308:C289-96 (2015). Read more (PubMed: 25428883) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34 cells)
Loading amount
5 µg
Specification
NSC34 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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