概述

  • 产品名称Anti-TRA2B抗体
    参阅全部 TRA2B 一抗
  • 描述
    兔多克隆抗体to TRA2B
  • 经测试应用适用于: CLIP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TRA2B.

    (Peptide available as ab32689.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Hela, Jurkat, A431, HEK293 This antibody gave a positive signal in the following methanol fixed cell line: HeLa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
CLIP Use at an assay dependent concentration. PubMed: 22194695
IHC-P 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). Although the predicted molecular mass of TRA2B is ~33 kDa, the available literature describes TRA2B running at ~40 kDa in SDS-PAGE
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-TRA2B antibody 图像

  • ICC/IF image of ab31353 stained HeLa cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31353 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Image courtesy of Human Protein Atlas . ab31353 staining TRA2B in human pancreas, showing a distinct and strong nuclear staining pattern in both exocrine pancreas and islet cells. Paraffin embedded human pancreas tissue was incubated with ab31353 (1/300 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab31353 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

  • All lanes : Anti-TRA2B antibody (ab31353) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
    Although the predicted molecular mass of TRA2B is ~33 kDa, the available literature describes TRA2B running at ~40 kDa in SDS-PAGE.
  • Anti-TRA2B antibody (ab31353) at 1/100 dilution + NSC34 mouse cell lysate at 20 µg

    Secondary
    IRDYE® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse testis tissue, staining TRA2B with ab31353. Staining was detected using DAB.

Anti-TRA2B antibody (ab31353)参考文献

This product has been referenced in:
  • Kuwano Y  et al. Transformer 2ß and miR-204 regulate apoptosis through competitive binding to 3' UTR of BCL2 mRNA. Cell Death Differ 22:815-25 (2015). Read more (PubMed: 25342468) »
  • Best A  et al. Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons. Nat Commun 5:4760 (2014). WB, CLIP ; Human . Read more (PubMed: 25208576) »

See all 11 Publications for this product

Product Wall

Thank you for your phone call.

I have contacted our datasheet department to look into the lot-dependent concentrations for this antibody and to have the datasheet updated. Thank you for pointing this out to us. I sincerely apologize for any p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (NSC34 cells)
Loading amount 20 µg
Specification NSC34 cells
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"