Anti-TR2-11抗体[449C1a] (ab58648)

概述

  • 产品名称Anti-TR2-11抗体[449C1a]
    参阅全部 TR2-11 一抗
  • 描述
    小鼠单克隆抗体[449C1a] to TR2-11
  • 经测试应用适用于: WB, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant N terminal fragment of Human TR2-11

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab58648 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • 克隆单克隆
  • 克隆编号449C1a
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 33 kDa (the immunising fragment) (predicted molecular weight: 53 kDa for full length protein).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 翻译后修饰Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression.
      Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
    • 细胞定位Nucleus. Nucleus > PML body. Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • NR2C 1 antibody
      • NR2C1 antibody
      • NR2C1_HUMAN antibody
      • NUCLEAR HORMONE RECEPTOR TR2 antibody
      • Nuclear receptor subfamily 2 group C member 1 antibody
      • Orphan nuclear receptor TR2 antibody
      • Testicular receptor 2 antibody
      • TR 2 antibody
      • TR2 antibody
      see all

    Anti-TR2-11 antibody [449C1a] 图像

    Anti-TR2-11 antibody [449C1a] (ab58648)参考文献

    ab58648 has not yet been referenced specifically in any publications.

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