概述

  • 产品名称Anti-TPX2抗体
    参阅全部 TPX2 一抗
  • 描述
    兔多克隆抗体to TPX2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 749) of Human TPX2 (Swiss-Prot Q9ULW0).

  • 阳性对照
    • HeLa whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/15000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
IP Use a concentration of 1 - 4 µg/ml.

靶标

  • 功能Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
  • 组织特异性Expressed in lung carcinoma cell lines but not in normal lung tissues.
  • 序列相似性Belongs to the TPX2 family.
  • 发展阶段Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C20ORF1 antibody
    • C20orf2 antibody
    • Chromosome 20 Open Reading Frame 1 antibody
    • Differentially expressed in cancerous and non-cancerous lung cells 2 antibody
    • Differentially expressed in cancerous and noncancerous lung cells 2 antibody
    • Differentially expressed in lung cells 2 antibody
    • Differentially expressed in lung cells antibody
    • DIL 2 antibody
    • DIL-2 antibody
    • DIL2 antibody
    • FLS353 antibody
    • GD:C20orf1 antibody
    • HCA 519 antibody
    • HCA519 antibody
    • HCTP 4 antibody
    • HCTP4 antibody
    • Hepatocellular carcinoma associated antigen 519 antibody
    • Hepatocellular carcinoma-associated antigen 519 antibody
    • p100 antibody
    • Preferentially expressed in colorectal cancer antibody
    • Protein fls353 antibody
    • REPP 86 antibody
    • repp86 antibody
    • Restricted expression proliferation associated protein 100 antibody
    • Restricted expression proliferation-associated protein 100 antibody
    • Targeting protein for Xklp2 antibody
    • TPX 2 antibody
    • TPX2 antibody
    • TPX2 microtubule associated homolog antibody
    • TPX2 microtubule associated protein homolog antibody
    • TPX2_HUMAN antibody
    see all

Anti-TPX2 antibody 图像

  • All lanes : Anti-TPX2 antibody (ab70237) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 5 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-TPX2 antibody (ab70237)参考文献

This product has been referenced in:
  • Hebras C & McDougall A Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos. PLoS One 7:e45431 (2012). ICC/IF . Read more (PubMed: 23029005) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Xenopus laevis cytoplasmic egg extract)
Specification Xenopus laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2011

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Xenopus laevis and tropicalis cytoplasmic egg extr)
Loading amount 40 µg
Specification Xenopus laevis and tropicalis cytoplasmic egg extr
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"