The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/15000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
Use a concentration of 1 - 4 µg/ml.
功能Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
组织特异性Expressed in lung carcinoma cell lines but not in normal lung tissues.
序列相似性Belongs to the TPX2 family.
发展阶段Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
Hebras C & McDougall A Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos. PLoS One7:e45431 (2012).
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