概述

  • 产品名称
  • 描述
    山羊多克隆抗体to TPTE
  • 特异性
    This antibody is expected to recognise all reported isoforms (NP_954870.2, NP_954868.1 and NP_954869.1). It may cross-react with the almost identical TPTE2 (GeneID 93492; NP_570141.2, NP_954864.1, NP_954863.1).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    ERRTDKTHSEKFQ

    (Human) from the internal region of the protein sequence according to NP_954870.2; NP_954868.1; NP_954869.1.

  • 阳性对照
    • Human testis lysates

性能

应用

Our Abpromise guarantee covers the use of ab77194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Could be involved in signal transduction.
    • 组织特异性
      Exclusively expressed in testis.
    • 序列相似性
      Contains 1 C2 tensin-type domain.
      Contains 1 phosphatase tensin-type domain.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cancer/testis antigen 44 antibody
      • CT44 antibody
      • EC=3.1.3.48 antibody
      • HGNC:12023 antibody
      • PTEN2 antibody
      • Putative tyrosine protein phosphatase antibody
      • Putative tyrosine-protein phosphatase TPTE antibody
      • TPTE antibody
      • TPTE_HUMAN antibody
      • Transmembrane phosphatase with tensin homology antibody
      • Tumor antigen BJ HCC 5 antibody
      • Tumor antigen BJ-HCC-5 antibody
      see all

    图片

    实验方案

    文献

    ab77194 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Recombinant protein (HEK293 cells expressing mouse Tpte protein)
    Loading amount
    20 µg
    Specification
    HEK293 cells expressing mouse Tpte protein
    Treatment
    CaPO4 transfected with mouse Tpte for 72 hours
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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