WB more details
Mouse, Cow, Dog
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TPST1.
This antibody gave a positive signal in HeLa and HepG2 whole cell lysates as well as in the following tissue lysates: Human lung; Mouse cerebellum; Rat cerebellum.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
功能 Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
序列相似性 Belongs to the protein sulfotransferase family.
细胞定位 Golgi apparatus membrane.
Information by UniProt
Protein tyrosine sulfotransferase 1 antibody
Protein-tyrosine sulfotransferase 1 antibody
Anti-TPST1 antibody 图像
Western blot - TPST1 antibody (ab100972)
All lanes : Anti-TPST1 antibody (ab100972) at 1 µg/ml Lane 1 : Lung (Human) Tissue Lysate Lane 2 : Cerebellum Mouse Tissue Lysate Lane 3 : Cerebellum Rat Tissue Lysate Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 42 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?) Additional bands at : 27 kDa,36 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"