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Mouse, Cow, Dog
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TPST1.
This antibody gave a positive signal in HeLa and HepG2 whole cell lysates as well as in the following tissue lysates: Human lung; Mouse cerebellum; Rat cerebellum.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
功能 Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
序列相似性 Belongs to the protein sulfotransferase family.
细胞定位 Golgi apparatus membrane.
Information by UniProt
Protein tyrosine sulfotransferase 1 antibody
Protein-tyrosine sulfotransferase 1 antibody
Anti-TPST1 antibody 图像
Western blot - TPST1 antibody (ab100972)
All lanes : Anti-TPST1 antibody (ab100972) at 1 µg/ml Lane 1 : Lung (Human) Tissue Lysate Lane 2 : Cerebellum Mouse Tissue Lysate Lane 3 : Cerebellum Rat Tissue Lysate Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 42 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?) Additional bands at : 27 kDa,36 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
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