重组Anti-TPR抗体[EPR8982] (ab170940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8982] to TPR
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TPR抗体[EPR8982]
参阅全部 TPR 一抗 -
描述
兔单克隆抗体[EPR8982] to TPR -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human TPR (Cysteine residue). The exact sequence is proprietary.
Database link: P12270 -
阳性对照
- HeLa + Camptothecin, HeLa, HeLa + Staurosporine, HeLa, 293T and Jurkat whole cell lysate (ab7899), HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8982 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170940于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 267 kDa.
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说明 |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/5000. Predicted molecular weight: 267 kDa. |
靶标
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功能
Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint. -
组织特异性
Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney. -
疾病相关
Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 7175 Human
- Omim: 189940 Human
- SwissProt: P12270 Human
- Unigene: 279640 Human
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别名
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
图片
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All lanes : Anti-TPR antibody [EPR8982] (ab170940) at 1/1000 dilution
Lane 1 : HeLa + Camptothecin cell lysates
Lanes 2 & 4 : HeLa cell lysates
Lane 3 : HeLa + Staurosporine cell lysates
Lane 5 : 293T cell lysates
Lane 6 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 267 kDa -
Immunofluorescence analysis of HeLa cells labeling TPR with ab170940 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab170940 被引用在 3 文献中.
- Li K et al. Stabilization of oncogenic transcripts by the IGF2BP3/ELAVL1 complex promotes tumorigenicity in colorectal cancer. Am J Cancer Res 10:2480-2494 (2020). PubMed: 32905413
- Wan J et al. Inhibitory effect of Ubenimex combined with fluorouracil on multiple drug resistance and P-glycoprotein expression level in non-small lung cancer. J Cell Mol Med 24:12840-12847 (2020). PubMed: 32945069
- Myers KN et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . PubMed: 27739501