概述

  • 产品名称Anti-TPR抗体
    参阅全部 TPR 一抗
  • 描述
    兔多克隆抗体to TPR
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 2300-2349 of Human TPR (NP_003283.1).

  • 阳性对照
    • Human Breast Carcinoma, Colon Carcinoma, Hodgkins Lymphoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma and mouse Squamous Cell Carcinoma tissue.
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

应用

Our Abpromise guarantee covers the use of ab84516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/500. Permeabilization with Triton-X 100 is recommended.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
    • 组织特异性Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
    • 疾病相关Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
      Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Nucleoprotein TPR antibody
      • tpr antibody
      • TPR_HUMAN antibody
      • translocated promoter region (to activated MET oncogene) antibody

    Anti-TPR antibody 图像

    • ab84516 at a 1/250 dilution, staining in human TPR in human prostate carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
    • ab84516 at a 1:250 dilution, staining mouse TPR in mouse squamous cell carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
    • ab84516 at a 1/250 dilution, staining human TPR in NBF-fixed asynchronous HeLa cells by immunocytochemistry. Detection: anti-rabbit IgG-FITC conjugate used at a dilution of 1/100.
    • ab84516 at a 1/250 dilution, staining TPR in human breast carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.

    Anti-TPR antibody (ab84516)参考文献

    This product has been referenced in:
    • Guo Y  et al. Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins. Mol Biol Cell 25:1287-97 (2014). ICC/IF ; Mouse . Read more (PubMed: 24523288) »
    • Umlauf D  et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci 126:2656-67 (2013). Human . Read more (PubMed: 23591820) »

    See all 2 Publications for this product

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