概述

  • 产品名称Anti-TPR抗体
    参阅全部 TPR 一抗
  • 描述
    兔多克隆抗体to TPR
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1650 and 1700 of human TPR

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab70610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
  • 组织特异性Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
  • 疾病相关Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
    Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nucleoprotein TPR antibody
    • tpr antibody
    • TPR_HUMAN antibody
    • translocated promoter region (to activated MET oncogene) antibody

Anti-TPR antibody 图像

  • All lanes : Anti-TPR antibody (ab70610) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 267 kDa
    Observed band size : 300 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 235 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human TPR by Western Blot of Immunprecipitate. ab70610 at 1µg/ml staining TPR in HeLa whole cell lysates immunoprecipitated using ab70610 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.

Anti-TPR antibody (ab70610)参考文献

ab70610 has not yet been referenced specifically in any publications.

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