antigen sequence, corresponding to amino acids 8-150 of Human TPPP2.
- Human kidney tissue; TPPP2 transfected HEK293T lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TPPP2 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-TPPP2 antibody (ab121215)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121215 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP2 antibody (ab121215)
ab121215 at 1/150 dilution staining TPPP2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Western blot - Anti-TPPP2 antibody (ab121215)
All lanes : Anti-TPPP2 antibody (ab121215) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (Coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells
Anti-TPPP2 antibody (ab121215)参考文献
has not yet been referenced specifically in any publications.
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