Anti-TPPP2抗体(ab121215)
Key features and details
- Rabbit polyclonal to TPPP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TPPP2抗体
参阅全部 TPPP2 一抗 -
描述
兔多克隆抗体to TPPP2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 8-150 of Human TPPP2.
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阳性对照
- Human kidney tissue; TPPP2 transfected HEK293T lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May bind tubulin but has no microtubule bundling activity. -
序列相似性
Belongs to the TPPP family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 122664 Human
- SwissProt: P59282 Human
- Unigene: 406966 Human
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别名
- C14orf8 antibody
- Gm1790 antibody
- Gm77 antibody
see all
图片
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121215 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121215 at 1/150 dilution staining TPPP2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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All lanes : Anti-TPPP2 antibody (ab121215) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (Coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121215 被引用在 2 文献中.
- Zhu F et al. PRSS55 plays an important role in the structural differentiation and energy metabolism of sperm and is required for male fertility in mice. J Cell Mol Med 25:2040-2051 (2021). PubMed: 33417308
- Zhu F et al. Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice. J Cell Mol Med 23:2583-2594 (2019). PubMed: 30680919