Anti-TPP1抗体(ab88305)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to TPP1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human TPP1 protein (NP_000382.3).

  • 阳性对照
    • WB: Human spleen tissue lysate, Jurkat cell lysate and TPP1 transfected 293T cell lysate IHC-P: human spleen tissue

性能

应用

Our Abpromise guarantee covers the use of ab88305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.

靶标

  • 功能
    Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
  • 组织特异性
    Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
  • 疾病相关
    Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
  • 序列相似性
    Belongs to the peptidase S53 family.
  • 翻译后修饰
    Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
  • 细胞定位
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell growth inhibiting gene 1 protein antibody
    • Cell growth-inhibiting gene 1 protein antibody
    • Ceroid lipofuscinosis neuronal 2 antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile (Jansky Bielschowsky disease) antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile antibody
    • CLN 2 antibody
    • CLN2 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • Growth inhibiting protein 1 antibody
    • LPIC antibody
    • Lysosomal pepstatin insensitive protease antibody
    • Lysosomal pepstatin-insensitive protease antibody
    • MGC21297 antibody
    • TPP 1 antibody
    • TPP I antibody
    • TPP-1 antibody
    • TPP-I antibody
    • Tpp1 antibody
    • TPP1_HUMAN antibody
    • TPPI antibody
    • Tripeptidyl aminopeptidase antibody
    • Tripeptidyl peptidase I antibody
    • Tripeptidyl-peptidase 1 antibody
    • Tripeptidyl-peptidase I antibody
    see all

图片

  • Anti-TPP1 antibody (ab88305) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-TPP1 antibody (ab88305) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TPP1 antibody (ab88305) at 1 µg/ml

    Lane 1 : TPP1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • ab88305, at 3 µg/ml, staining TPP1 in formalin fixed, paraffin embedded human spleen sections by Immunohistochemistry (Immunoperoxidase).

文献

ab88305 has not yet been referenced specifically in any publications.

客户评价及客户问答

The TTP1 antibody ab88305 was generated against full-length human protein, not a peptide. Alignment of the human and canine TTP1 shows 90% identity, using the sequences at these UniProt pages,


Canine http://www.uniprot.org/uniprot/Q9XSB8<...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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