概述
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产品名称Anti-TPOR抗体[EPR4194]
参阅全部 TPOR 一抗 -
描述兔单克隆抗体[EPR4194] to TPOR
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TPOR aa 600 to the C-terminus.
Database link: P40238 -
阳性对照
- HepG2, A431, and Human marrow lysates.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EPR4194
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab109003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 71 kDa. |
靶标
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功能Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
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组织特异性Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
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疾病相关Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM:604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
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序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 4352 Human
- Entrez Gene: 17480 Mouse
- Entrez Gene: 366455 Rat
- Omim: 159530 Human
- SwissProt: P40238 Human
- SwissProt: Q08351 Mouse
- Unigene: 82906 Human
- Unigene: 4864 Mouse
see all -
别名
- C MPL antibody
- CD110 antibody
- mpl antibody
see all
图片
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![Western blot - Anti-TPOR antibody [EPR4194] (ab109003)](http://www.abcam.cn/ps/products/109/ab109003/Images/ab109003-254461-ab109003WB3.jpg)
Western blot - Anti-TPOR antibody [EPR4194] (ab109003)Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilution (purified) + mouse spleen lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 82 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-TPOR antibody [EPR4194] (ab109003)](http://www.abcam.cn/ps/products/109/ab109003/Images/ab109003-254460-ab109003WB2.jpg)
Western blot - Anti-TPOR antibody [EPR4194] (ab109003)All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/5000 dilution (purified)
Lane 1 : K562 cell lysate
Lane 2 : rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 82 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-TPOR antibody [EPR4194] (ab109003)](http://www.abcam.cn/ps/products/109/ab109003/Images/ab109003-254459-ab109003WB1.jpg)
Western blot - Anti-TPOR antibody [EPR4194] (ab109003)Anti-TPOR antibody [EPR4194] (ab109003) at 1/2500 dilution (purified) + A431 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 82 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-TPOR antibody [EPR4194] (ab109003)](http://www.abcam.cn/ps/products/109/ab109003/Images/TPOR-Primary-antibodies-ab109003-2.JPG)
Western blot - Anti-TPOR antibody [EPR4194] (ab109003)All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human marrow lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 71 kDa
文献
ab109003 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"