Anti-TPMT抗体[AT2E7] (ab122984)

概述

  • 产品名称
    Anti-TPMT抗体[AT2E7]
    参阅全部 TPMT 一抗
  • 描述
    小鼠单克隆抗体[AT2E7] to TPMT
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-245 of Human TPMT purified from E. coli (NP_000358).

  • 阳性对照
    • Hep3B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab122984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
  • 疾病相关
    Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.
  • 序列相似性
    Belongs to the methyltransferase superfamily. TPMT family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HGNC:12014 antibody
    • S adenosyl L methionine thiopurine S methyltransferase antibody
    • Thiopurine methyltransferase antibody
    • Thiopurine S methyltransferase antibody
    • Thiopurine S-methyltransferase antibody
    • TPMT antibody
    • TPMT_HUMAN antibody
    see all

图片

  • Anti-TPMT antibody [AT2E7] (ab122984) at 1/1000 dilution + Hep3B lysate at 40 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 28 kDa


  • Predicted band size : 28 kDa

    The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : Recombinant Protein
    Lane 2 : HepG2 cell lysate

  • Immunocytochemistry/Immunofluorescence analysis of U87MG cells labelling TPMT with ab122984 at 1/100. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry analysis of U87MG cells labelling TPMT with ab122984, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

文献

ab122984 has not yet been referenced specifically in any publications.

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