Anti-TPH2抗体(ab121020)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to TPH2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide:

    C-SLTQNKAIKSEDK

    , corresponding to internal sequence amino acids 33-45 of Mouse TPH2 (NP_775567.2; Q8CGV2).

  • 阳性对照
    • Mouse fetal brain lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field.
IHC-P Use a concentration of 0.5 - 2 µg/ml.

靶标

  • 组织特异性
    Brain specific.
  • 通路
    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 疾病相关
    Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • 序列相似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Anti-TPH2 antibody 图像

  • IHC-P image of THP2 staining on mouse sections of Substantia Nigra using ab121020 (1:800). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121020 was diluted 1:800and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.

  • ab121020 staining TPH2 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse Brain stem shows staining of Raphe nucleii serotonergic neurons: Left = sagittal section, Right = coronal section. Antibody diluted to 0.5 μg/ml.

Anti-TPH2 antibody (ab121020)参考文献

ab121020 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate 80ºC
Permeabilization
Yes - 0.25% Triton in PBS
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Teodora Georgescu

Verified customer

提交于 Jul 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册