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Our Abpromise guarantee covers the use of ab111828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunoprecipitation analysis of Raphe Nuclei isolated from Sprague-Dawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.
Lane 1 (Positive Control): Non-immunoprecipitated brain lysates were probed with ab111828.
Lane 2 (ab111828 Ms TPH2): Immunoprecipitated Proteins
Lane 3 (Sheep TPH2 antibody): Immunoprecipitated Proteins
Lane 4 (Negative Control => Anti-Tyrosine Hydroxylase antibody): Immunoprecipitated proteins
ab111828 staining TPH2 in Mouse dorsal raphe coronal brain slices tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 2% serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/800 in PBS + Triton X-100 + serum) at 22°C for 24 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500 in PBS + Goat serum) was used as the secondary antibody.
ab111828 has not yet been referenced specifically in any publications.