Anti-TPH2抗体(ab111828)

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概述

  • 产品名称Anti-TPH2抗体
    参阅全部 TPH2 一抗
  • 描述
    兔多克隆抗体to TPH2
  • 经测试应用适用于: WB, IP, ICC, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Non human primates
  • 免疫原

    Synthetic peptide:

    RRGLSLDSAVPEDHQL

    , corresponding to N terminal amino acids 15-30 of Human TPH2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP 1/1000.
ICC 1/500.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 组织特异性Brain specific.
  • 通路Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 疾病相关Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Anti-TPH2 antibody 图像

  • Immunoprecipitation - Anti-TPH2 antibody (ab111828)
    Immunoprecipitation - Anti-TPH2 antibody (ab111828)

    Immunoprecipitation analysis of Raphe Nuclei isolated from Sprague-Dawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.

     

    Lane 1 (Positive Control): Non-immunoprecipitated brain lysates were probed with ab111828.
    Lane 2 (ab111828 Ms TPH2): Immunoprecipitated Proteins
    Lane 3 (Sheep TPH2 antibody): Immunoprecipitated Proteins
    Lane 4 (Negative Control => Anti-Tyrosine Hydroxylase antibody): Immunoprecipitated proteins

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TPH2 antibody (ab111828)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TPH2 antibody (ab111828)This image is courtesy of an Abreview submitted by Jason Teem

    ab111828 staining TPH2 in Mouse dorsal raphe coronal brain slices tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 2% serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/800 in PBS + Triton X-100 + serum) at 22°C for 24 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500 in PBS + Goat serum) was used as the secondary antibody.

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Anti-TPH2 antibody (ab111828)参考文献

ab111828 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab111828？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TPH2 antibody

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Dorsal Raphe coronal brain slices)
Specification Dorsal Raphe coronal brain slices
Permeabilization Yes - TritonX-100, 0.3%
Fixative Paraformaldehyde
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Jason Teem

Verified customer

提交于 Nov 04 2013

Thank you for getting back in touch. The deal sounds great, please send through the discount code.

DISCOUNT CODE: *** Expiration date: April 21st, 2012 I am very pleased to hear you would like to use ab111828 in IHC and send us an image of your results. This code will give you 1 free primary antibody before the expiration date. To redeem this offer,...

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I am currently looking for an Anti-TPH2 antibody to use for an immunohistochemistry. I have been on your website and have seen that you do stock it, do you have any images of Anti-TPH2 having been used? If so, would it be possible to send through an im...

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Thank you very much for your interest in ab111828. Unfortunately, we would be unable to ship this antibody faster than 2-3 weeks, and we do not currently have an image of ab111828 in IHC. Therefore, I can offer a discount off a future purchase if you b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"