Anti-TPA组织Plasminogen Activator抗体[T-1] (ab82249)

概述

  • 产品名称Anti-TPA组织Plasminogen Activator抗体[T-1]
    参阅全部 TPA Tissue Plasminogen Activator 一抗
  • 描述
    小鼠单克隆抗体[T-1] to TPA组织Plasminogen Activator
  • 经测试应用适用于: WB, IHC-Fr, ELISA, IP, Functional Studiesmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Native human TPA Tissue Plasminogen Activator

  • 表位ab82249 binds to the A chain of TPA Tissue Plasminogen Activator.

性能

应用

Our Abpromise guarantee covers the use of ab82249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 63 kDa.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Inhibits the enzymatic activity of TPA Tissue Plasminogen Activator. Can also be used for immunopurification

靶标

  • 功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 组织特异性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 结构域Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alteplase antibody
    • DKFZp686I03148 antibody
    • Plasminogen activator tissue antibody
    • Plasminogen activator tissue type antibody
    • PLAT antibody
    • Reteplase antibody
    • t PA antibody
    • T Plasminogen Activator antibody
    • t-PA antibody
    • T-plasminogen activator antibody
    • Tissue plasminogen activator (t PA) antibody
    • Tissue type plasminogen activator antibody
    • Tissue-type plasminogen activator chain B antibody
    • tPA antibody
    • TPA_HUMAN antibody
    • TPA1 antibody
    see all

Anti-TPA Tissue Plasminogen Activator antibody [T-1] (ab82249)参考文献

This product has been referenced in:
  • Lund LR  et al. Plasminogen activator inhibitor type 1: cell-specific and differentiation-induced expression and regulation in human cell lines, as determined by enzyme-linked immunosorbent assay. Mol Cell Endocrinol 60:43-53 (1988). ELISA . Read more (PubMed: 3265112) »
  • Grøndahl-Hansen J  et al. Immunohistochemical localization of urokinase- and tissue-type plasminogen activators in psoriatic skin. J Invest Dermatol 88:28-32 (1987). IHC ; Human . Read more (PubMed: 3098860) »

See all 4 Publications for this product

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Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during two weeks.

We recently undertook an in-depth study examining storage conditions and antibody perfo...

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Thank you for your inquiry.

Polyclonal antibodies are serum/plasma derived since they are taken from the blood of the animal. Monoclonal antibodies are generated by culturing the B cell clone that is producing the antibody in cell culture or ...

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/n not have a matching pair. However, I think we have one antibody which may interest you:

http://www.abcam.com/index.html?datasheet=82249 (or use the following: http://www.abcam.com/index.html?datasheet=82249).
This antibody is derived ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"