概述

  • 产品名称
    Anti-TOX4抗体
  • 描述
    兔多克隆抗体to TOX4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: MEFPGGNDNY LTITGPSHPF LSGAETFHTP SLGDEEFEIP PISLDSDPSL, corresponding to a region within N terminal amino acids 1-50 of Human TOX4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • TOX4 transfected 293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

靶标

图片

  • Western blot - TOX4 antibody (ab66651)
    Western blot - TOX4 antibody (ab66651)
    All lanes : Anti-TOX4 antibody (ab66651) at 1.25 µg/ml

    Lane 1 : Molecular Weight Markers
    Lane 2 : Transfected 293T lysate at 10 µg

    Secondary
    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 66 kDa
    Observed band size: 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab66651 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab66651？请让我们知道，以便我们可以引用本数据表中的参考文章。

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