Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
Synthetic peptide: MEFPGGNDNY LTITGPSHPF LSGAETFHTP SLGDEEFEIP PISLDSDPSL, corresponding to a region within N terminal amino acids 1-50 of Human TOX4
- TOX4 transfected 293T lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
Information by UniProt
- C14orf92 antibody
- Epidermal Langerhans cell protein LCP1 antibody
- KIAA0737 antibody
Western blot - TOX4 antibody (ab66651)
All lanes :
Anti-TOX4 antibody (ab66651) at 1.25 µg/mlLane 1 :
Molecular Weight MarkersLane 2 :
Transfected 293T lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
66 kDaObserved band size :
74 kDa (why is the actual band size different from the predicted?
)Additional bands at :
100 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"