The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/128000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 63 kDa.
Preliminary experiments gave an approx 100kDa band in Human Brain (Cerebellum) lysates after 0.2µg/ml antibody staining. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
Please note that currently we cannot find an explanation in the literature for the size of the band we observe.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.