Total OXPHOS人WB抗体Cocktail (ab110411)


  • 产品名称Total OXPHOS人WB抗体Cocktail
  • 检测类型Quantitative
  • 种属反应性
    与反应: Human
  • 产品概述

    ab110411  contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria (50 µg) are also included as a Western Blotting control.

    Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

    The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).

    The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.

    The five antibodies provided in the cocktail are all mouse monoclonal antibodies.

  • 说明

    Shipped  on Dry Ice.

    Store cocktail at 4°C and control at -80°C.

  • 经测试应用适用于: WBmore details


  • 别名
    • OXPHOS cocktail


Our Abpromise guarantee covers the use of ab110411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.

Total OXPHOS Human WB Antibody Cocktail 图像

  • This image is courtesy of an anonymous Abreview

    Gel: Non-reduced Denaturing (4-12%)

    Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.

    Blocked for 1 hour with 5% Milk at room temperature.

    ab110411 used as per instructions.

    Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300

    Detection method: ECL+ 2 minutes exposure.


  • Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
    ab110411(MS601) was used to label lane 6, and the individual antibodies
    comprising the cocktail were used for lanes 1-5.

Total OXPHOS Human WB Antibody Cocktail (ab110411)参考文献

This product has been referenced in:
  • Liwak-Muir U  et al. Impaired activity of CCA-adding enzyme TRNT1 impacts OXPHOS complexes and cellular respiration in SIFD patient-derived fibroblasts. Orphanet J Rare Dis 11:79 (2016). WB ; Human . Read more (PubMed: 27317422) »
  • Cheng CT  et al. Metabolic Stress-Induced Phosphorylation of KAP1 Ser473 Blocks Mitochondrial Fusion in Breast Cancer Cells. Cancer Res 76:5006-18 (2016). Read more (PubMed: 27364555) »

See all 104 Publications for this product

Product Wall

Thank you for contacting us.

The answer is yes, you can use reducing reagent in the sample but we do not recommend boiling samples since it will cause signal reducing of several bands.

I hope this information is helpful to you. Pl...

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Excellent Excellent 5/5 (Ease of Use)
MitoProfile Total OXPHOS
40 ug total protein from human heart tissue whole lysate
12% tris-glycine gel
5% milk-TBST block, 1 h, RT
primary of 1:750 in 5% milk for 16 h at 4C
secondary of 1:1250 in 5% milk for 1 h at RT
exposure varied based on band
excellent ease of use - 5/5
image attached

Abcam user community

Verified customer

提交于 Oct 29 2014

Thank you for your enquiry.

I am pleased to help answer your questions:

1. what do you mean by labile protein for the differents complexes? are they degraded or found in the cytoplasm?

Several of the proteins are degrad...

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Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

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The link below shows the genes in the mitochondrial DNA. This is a very comprehensive review.

There are 37 mitochondrial DNA (mtDNA)- encoded genes which include se...

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Total OXPHOS antibody cocktail

Excellent Excellent 5/5 (Ease of Use)
In this experiment we used isolated mitochondria from human HCT116 colon cancer cell line. The proteins from the mitochondrial lysate were seperated on a 13% SDS gel. The total OXPHOS human WB antibody kit was diluted 1:1000 in 2% milk. We already obtained strong signals of the complexes V and I after 10 sec. After 1 min (attached figure) we got signals from all five complexes.

The kit is easy to use and gives reproducibly results.

Mr. Christian Marx

Verified customer

提交于 Aug 21 2013

In-house we test this cocktail using isolated mitochondra, but other users have published data using whole cell or whole tissue homogenates. We have these publications listed on the datasheet-

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Thank you for your reply.

I can understand why you find the nomenclature a bit confusing and we will certainly strive in the future to make the nomenclature on our datasheets more consistent by ensuring that the full name is added to the targ...

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Thank you for your inquiry.

There is a representative protein from each of the 5 OXPHOS complexes in this mouse monoclonal antibody cocktail (ab110411, MS601).

I can confirm that one protein, COX-II (Uniprot P00403) is mtDNA encoded...

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Hello! A few more tips for troubleshooting!

Blue native (BNPAGE) doesn’t have great power of resolution (unlike SDSPAGE),t he main reason for this is that the complexes are in multiple states of assembly therefore some give multiple and ...

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