RabMAb

Anti-Torsin A抗体[EP2569Y] (ab76133)

概述

  • 产品名称Anti-Torsin A抗体[EP2569Y]
    参阅全部 Torsin A 一抗
  • 描述
    兔单克隆抗体[EP2569Y] to Torsin A
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminal of human Torsin A

  • 阳性对照
    • SH-SY5Y and 293T cell lysates. Permeabilized SH-SY5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Flow Cyt 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    Anti-Torsin A antibody [EP2569Y] 图像

    • All lanes : Anti-Torsin A antibody [EP2569Y] (ab76133) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,150 kDa. We are unsure as to the identity of these extra bands.
    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76133 at 1/150 dilution (red) or a rabbit IgG (negative) (green).

    Anti-Torsin A antibody [EP2569Y] (ab76133)参考文献

    ab76133 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"