概述

  • 产品名称Anti-Torsin A抗体
    参阅全部 Torsin A 一抗
  • 描述
    兔多克隆抗体to Torsin A
  • 特异性The immunogen used to raise this antibody has 92% homology with the human Torsin A protein. Some customers have successfully used ab34540 in human tissue. Please contact Abcam Scientific Support for more information.
  • 经测试应用适用于: IHC-FoFr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Rat Torsin A.

    (Peptide available as ab35510.)

  • 阳性对照
    • This antibody gave a positive control in the following mouse lysates: Brain tissue Testis tissue Heart tissue Skeletal Muscle tissue Liver tissue Mouse Fetal lysate - 14 Days old Tissue Brain Tissue Lysate - normal tissue, 0 days old NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell MEF1 (Mouse embryonic fibroblast cell line) Whole Cell This antibody gave a positive control in the following rat lysates: Liver tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab34540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/100.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

靶标

Anti-Torsin A antibody 图像

  • All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Mouse Brain at 10 µg
    Lane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
    Lane 4 : Mouse Heart Mouse Tissue Lysate at 10 µg
    Lane 5 : Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
    Lane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
    Lane 7 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
    Lane 8 : Mouse Embryonic Fibroblast 1 at 10 µg
    Lane 9 : NIH 3T3 whole cell lysate (ab7179) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 105 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC-FoFr image of Torsin A (ab34540) on Rat Cerebral Cortex section. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-Torsin A antibody (ab34540)参考文献

This product has been referenced in:
  • Yokoi F  et al. Behavioral and electrophysiological characterization of Dyt1 heterozygous knockout mice. PLoS One 10:e0120916 (2015). WB ; Mouse . Read more (PubMed: 25799505) »
  • Zhang L  et al. Dopamine receptor and Ga(olf) expression in DYT1 dystonia mouse models during postnatal development. PLoS One 10:e0123104 (2015). WB . Read more (PubMed: 25860259) »

See all 15 Publications for this product

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Thank you for your enquiry.

The exact immunogen sequence for ab34540 is as follows:

CKTVFTKLDYYLDD

I am sorry to confirm this has not been specifically tested for cross reactivity to TOR1B. However, I have conducted a B...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain - Cerebral Cortex)
Specification Brain - Cerebral Cortex
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 07 2010

Application Western blot
Sample Human Cell lysate - whole cell (HEK 293 cells transfected with human Torsin A)
Loading amount 50 µg
Specification HEK 293 cells transfected with human Torsin A
Gel Running Conditions Reduced Denaturing (10%)
Blocking step LI-COR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"