Synthetic peptide corresponding to an unique epitope on the Torc2 protein. The peptide was covalently modified post synthetically and covalently coupled to a carrier protein for desired antigenicity before injecting in to rabbits.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium azide
The antiserum is supplied in antibody stabilization buffer containing Sodium Azide or Thimerosal.
Concentration information loading...
纯度Immunogen affinity purified
纯化说明The antibody was affinity purified over immobilized antigen based chromatography, and the purified immunoglobulin was stabilized in antibody stabilization buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 73 kDa). The antibody also labels a 55 kDa protein believed to be a smaller variant of Torc2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
组织特异性Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
序列相似性Belongs to the TORC family.
翻译后修饰Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
细胞定位Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.