Anti-TORC1抗体[EPR3382] (ab92477)


  • 产品名称Anti-TORC1抗体[EPR3382]
    参阅全部 TORC1 一抗
  • 描述
    兔单克隆抗体[EPR3382] to TORC1
  • 经测试应用适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MECT1.

  • 阳性对照
    • Fetal brain and C6 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab92477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 67 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
    • 组织特异性Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
    • 疾病相关A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
    • 序列相似性Belongs to the TORC family.
    • 翻译后修饰Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
    • 细胞定位Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA0616 antibody
      • CREB regulated transcription coactivator 1 antibody
      • CREB-regulated transcription coactivator 1 antibody
      • CRTC1 antibody
      • CRTC1_HUMAN antibody
      • FLJ14027 antibody
      • MECT 1 antibody
      • Mucoepidermoid carcinoma translocated 1 antibody
      • Mucoepidermoid carcinoma translocated protein 1 antibody
      • TORC-1 antibody
      • TORC1 antibody
      • Transducer of CREB protein 1 antibody
      • Transducer of regulated cAMP response element binding protein 1 antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
      • Transducer of regulated cAMP response element-binding protein 1 antibody
      • WAMTP1 antibody
      see all

    Anti-TORC1 antibody [EPR3382] 图像

    • Overlay histogram showing SHSY-5Y cells stained with ab92477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92477, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    Anti-TORC1 antibody [EPR3382] (ab92477)参考文献

    ab92477 has not yet been referenced specifically in any publications.

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