Anti-TORC1抗体(ab115330)

概述

  • 产品名称Anti-TORC1抗体
    参阅全部 TORC1 一抗
  • 描述
    兔多克隆抗体to TORC1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Zebrafish
  • 免疫原

    Synthetic peptide:

    QKQAEETAAFEEVMKD

    conjugated to KLH, corresponding to amino acids 19-34 of Human MECT1.

  • 阳性对照
    • Human Kidney tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab115330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 67 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000 - 1/40000.

靶标

  • 功能Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
  • 组织特异性Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
  • 疾病相关A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
  • 序列相似性Belongs to the TORC family.
  • 翻译后修饰Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0616 antibody
    • CREB regulated transcription coactivator 1 antibody
    • CREB-regulated transcription coactivator 1 antibody
    • CRTC1 antibody
    • CRTC1_HUMAN antibody
    • FLJ14027 antibody
    • MECT 1 antibody
    • Mucoepidermoid carcinoma translocated 1 antibody
    • Mucoepidermoid carcinoma translocated protein 1 antibody
    • TORC-1 antibody
    • TORC1 antibody
    • Transducer of CREB protein 1 antibody
    • Transducer of regulated cAMP response element binding protein 1 antibody
    • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
    • Transducer of regulated cAMP response element-binding protein 1 antibody
    • WAMTP1 antibody
    see all

Anti-TORC1 antibody 图像

  • ab115330, at 1/500 dilution, staining MECT1 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TORC1 antibody (ab115330)参考文献

ab115330 has not yet been referenced specifically in any publications.

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According to Swiss Prot and publications (http://www.ncbi.nlm.nih.gov/pubmed?db=pubmed&cmd=search&term=17334997&dopt=b), the break point for the translocation to form the CRTC1-MAML2/MAML2-CRTC1 fusion...

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We are not aware if any of our antibodies to TORC1 (CRTC1) have been involved in such scientific investigations. If they were, then the publication list of the on-line datasheet wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"