Anti-TOR1AIP1抗体(ab86307)

概述

  • 产品名称Anti-TOR1AIP1抗体
    参阅全部 TOR1AIP1 一抗
  • 描述
    兔多克隆抗体to TOR1AIP1
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 175 and 225 of human TOR1AIP1 (NP_056417.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab86307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
IP
  • 应用说明IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 66 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
    • 序列相似性Belongs to the TOR1AIP family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Lamin-associated protein 1B antibody
      • Lamina associated polypeptide 1B antibody
      • Lamina associated polypeptide 1C antibody
      • LAP1 antibody
      • LAP1B antibody
      • TOIP1_HUMAN antibody
      • TOR1AIP1 antibody
      • Torsin A interacting protein 1 antibody
      • Torsin-1A-interacting protein 1 antibody
      see all

    Anti-TOR1AIP1 antibody 图像

    • All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 66 kDa
      Observed band size : 66 kDa


      Exposure time : 30 seconds
    • Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
      ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-TOR1AIP1 antibody (ab86307)参考文献

    ab86307 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - CSK buffer, 5 min at RT after fixing
    Fixative Paraformaldehyde
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    提交于 Sep 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"