Anti-Topoisomerase III alpha抗体(ab108493)


  • 产品名称Anti-Topoisomerase III alpha抗体
    参阅全部 Topoisomerase III alpha 一抗
  • 描述
    兔多克隆抗体to Topoisomerase III alpha
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human Topoisomerase III alpha.

  • 阳性对照
    • This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab108493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
  • 组织特异性High expression is found in testis, heart, skeletal muscle and pancreas.
  • 序列相似性Belongs to the prokaryotic type I/III topoisomerase family.
    Contains 1 Toprim domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA topoisomerase 3 alpha antibody
    • DNA topoisomerase 3-alpha antibody
    • DNA topoisomerase III alpha antibody
    • TOP 3 antibody
    • TOP3 antibody
    • TOP3A antibody
    • TOP3A_HUMAN antibody
    • topo III-alpha antibody
    • topoisomerase (DNA) III alpha antibody
    • topoisomerase (DNA) III antibody
    • topoisomerase III alpha antibody
    • ZGRF7 antibody
    • zinc finger, GRF-type containing 7 antibody
    see all

Anti-Topoisomerase III alpha antibody 图像

  • All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Topoisomerase III alpha antibody (ab108493)参考文献

ab108493 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108493.
Please use the links above to contact us or submit feedback about this product.